at Peking University School of Pharmaceutical Sciences
​​​​the dong research group
4. An Advance in Proline Ligation
J. Am. Chem. Soc. 2011, 133, 10784-10786.
Shiying Shang, Zhongping Tan, Suwei Dong, and Samuel J. Danishefsky*

Native chemical ligation (NCL) is widely applicable for building proteins in the laboratory. Since the discovery of this method, many strategies have been developed to enhance its capability and efficiency. Because of the poor reactivity of proline thioesters, ligation at a C-terminal proline site is not readily accomplished. Here, we demonstrate that ligation at an N-terminal protein is feasible using the combined logic of NCL and metal-free dethiylation (MFD).