5. Toward Homogeneous Erythropoietin: Application of Metal‐Free Dethiylation in the Chemical Synthesis of the Ala79‐Arg166 Glycopeptide Domain
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作者: Suwei Dong, Shiying Shang, Zhongping Tan, Samuel J. Danishefsky*
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发布时间: 2011-10-29
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Israel J. Chem. 2011, 51, 968-976.
Suwei Dong, Shiying Shang, Zhongping Tan, Samuel J. Danishefsky*
We describe herein the assembly of hEPO(79–166), a key glycopeptide segment en route to erythropoietin, in minimally protected form. Key to the success of this synthetic endeavor was the application of our two‐step cysteine‐free native chemical ligation strategy, by which we achieved formal ligation at alanine and proline residues through the use of an N‐terminal amino acid surrogate presenting a readily removable thiol functionality.
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